Pcr Cycle Graph. Explain what the four lanes of your gel would look like. Amplification curve crosses the threshold to where it meets the x axis of the graph corresponding to the ct value.
As with the initial template dna denaturation step the time and temperature should be optimized according to the nature of the template dna dna polymerase and buffer components. The pcr test uses amplification cycles to find viral rna. Each of these polymerase chain reaction steps is repeated 30 40 times cycles.
Defend your answer with evidence from the graph.
Explain what the four lanes of your gel would look like. An overview of cycle threshold values and. Show the bands you expect to see on the empty gel image. Each cycle of amplification doubles the number of molecules in a sample.
